![]() ![]() Compare the linear ranges to determine the amount of sample you should load to produce a linear response for both target and control. This range must be determined individually for the target protein and internal loading control. This protocol is intended for use with near-infrared fluorescent Western blots. This protocol explains how to use serial dilutions of sample protein to determine the linear ranges of detection for a target and internal loading control, and choose an appropriate amount of sample to load for QWB analysis. Increasing amounts of target do not produce a proportional increase in the recorded signal.
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